Date: Mon, 29 Dec 2003 11:18:21 -0500
From: Valerie Pence <valerie.pence@cincinnatizoo.org>
To: 'bryonet-l@mtu.edu' <bryonet-l@mtu.edu>
BRYONET
We have had some success using Benlate in the medium (100mg/L). There's
alwyas a tenuous balance between sterilizing procedures/antimicrobial agents
in the medium killing the microbes and killing the tissues. I would suggest
also trying different lengths of time for the surface sterilization--again,
you need long enough to kill the microbes and short enough not to kill the
plant tissues. And, try doing a number of pieces with each treatment.
Usually, when we have something "come through" the process and establish an
in vitro culture, it's one small piece that lived while others died, or one
small piece that was clean while the others were contaminated.
Good luck.
Valerie Pence
Center for Conservation and Research of Endangered Wildlife
Cincinnati Zoo and Botanical Garden
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