To: bryonet-l@mtu.edu
Date: Thu, 9 Nov 2000 09:16:29 -0000
BRYONET
Sorry, I haven't been following the discussion, so this may have
already been said, but while carrying out a PhD on protonema, I
experimented with trying to get a number of species into axenic
culture.
First off was Tetraphis pellucida, which was easy to culture
axenically from the gemmae. I washed these onto a filter paper
disc using 5% hypochlorite with a little wetting agent, and then
fished them off onto agar using a tungsten needle under a binocular.
Other genera were also successfully cultured from gemmae in this
way, including Aulocomnium and Ullota.
However, the easiest and most reliable way of culturing bryophytes
is from using spores. Disinfect intact spore capsules and crush
them into sterile distilled water, and then inoculate the agar plates.
Bingo!
Although vegetative material regenerates readily, I was
unsuccessful in getting sterile cultures from other bits of mosses.
With best regards
Jamie Goode PhD
Editor
Novartis Foundation
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