Date: Thu, 9 Nov 2000 08:31:24 -0500
From: Randy Cameron <rcameron@citrus.usda.gov>
To: <bryonet-l@mtu.edu>
BRYONET
I was able to grow several species of Splachnum in axenic culture on
sterilized washed sand using sterilized Bold's Basal Medium as a nutrient
source. The cultures were initiated by inoculating them with spores from
capsules that had been surface sterilized and then extruding the spores into
a drop of sterile water by rolling a sterile probe over the side of
capsule, forcing the spores into the sterile water. The spores were then
surface sterilized before transferring them to the sand. I did have an
occasional problem with fungal contamination but was able to grow most
cultures through gametangia formation. I tried a few times to flood the
cultures to release sperm and iniate sporophyte production but wasn't very
sucessful, although I really didn't put a lot of effort into that aspect.
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