Date: Thu, 3 Jul 2003 11:28:08 -0700
From: Curt Seeliger <seeliger.curt@epa.gov>
To: bryonet-l@mtu.edu
BRYONET
Karla,
You might want to track down a mycorrhiza researcher as they often have
the same concerns as you do.
Sand sterilization isn't that difficult if you have an autoclave, but
keeping it sterile will be problematic. If that's important, you might
want to include a few containers with no Fissidens, but which were
otherwise treated identically, to gauge how likely the moss cultures are
axenic.
Since you're interested in nutrients as well, you'll want to wash the sand
with some sort of acid to eluviate nutrients. My plant path. prof.
fairly giggled about this practice, as the acid releases nutrients from
the sand surface, leaving you with measurable quantities of available
minerals.
cur
> >Hello,
> >My name is Karla Werner. I am currently working on my masters in
> > bryology and am going to be studying the effects of nutrient
> > deficiencies in Fissidens. In order to do so, I need to grow the moss
> > on a medium that has no nutrients. If you have a suggestion or know
> > where I can buy sterile sand, please let me know.
> >Thank you.
--
Curt Seeliger, Data Ranger
CSC, EPA/WED contractor
541/754-4638
seeliger.curt@epa.gov
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