From: Rod Seppelt <Rod.Seppelt@aad.gov.au>
To: <bryonet-l@mtu.edu>
BRYONET
Various methods - Lewis Anderson's coffee grinder technique, buttermilk
in the slurry, yoghurt in the slurry - have been used and discussed from
time to time on BRYONET. What the chemistry of the "milk shake"
techniques is, remains open to debate. Perhaps it just makes the
fragments adhere to the substrate, perhaps there is some chemical growth
promoter. Egg albumin has been used in microscopy to adhere wax
embedded sections to a glass slide. That was replaced with Haupt's
adhesive (a gelatin, water, glycerin, phenol mix - the phenol is a
preservative) when I was last actively making teaching slides. Whatever
the technique used, if it works, then go for it.
Sean Edwards remarked on his success with a fine mist spray. Certainly,
keeping the fragments or propagules moist initially will be of great
benefit. But, if Sean can have success with tap water in England (high
in Ca, high in all sorts of chemical elements) then perhaps a green
finger also helps!!
Prof. Rod Seppelt,
Australian Antarctic Division
Channel Highway,
Kingston, Tasmania 7050,
Australia
ph: +61 (03) 62 323 438
FAX: +61 (03) 62 323 449
email: rod.seppelt@aad.gov.au
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