BRYONET: chromosome count of hornworts

BRYONET

Dear all,
I want to count the chromosome number of hornworts, but encounter two
obstacles now: both hydrolyzation and enzymes can not detach the thallus
cells; all the staining agents I used, including Carbol fuchsin and
aceto carmine, can not stain the nucleus. Any suggestions will be
welcome and thank you very much!
Qinghua Wang
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Institute of Botany
Chinese Academy of Sciences
Nanxincun, Xiangshan, Beijing 100093
P. R. China
email: qinghua2699@yahoo.com.cn
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________________________________________
http://card.mail.cn.yahoo.com/
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